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논문분류	춘계학술대회 초록집
제목	The Autophagy Enhancer MSL-7 Improves Renal Injury by Modulating of Autophagy-Lysosomal Pathway in Adenine-Induced Chronic Kidney Disease Mouse Model
저자	Jina Lee
출판정보	2026; 2026(1):
키워드	Chronic Kidney Disease, Autophagy, Lysosomal stress, Fibrosis
초록	Objectives: Autophagy–lysosomal pathway dysfunction induces intracellular protein accumulation and lysosomal stress, contributing to the progression of renal fibrosis in chronic kidney disease (CKD). In CKD kidneys, p62 accumulation and reduced lysosomal markers are considered representative features of impaired autophagy flux, and regulation of lysosomal homeostasis, including TFEB-related pathways, has emerged as a potential therapeutic strategy. This study evaluated whether MSL-7, a small-molecule autophagy enhancer, improves renal injury through modulation of the autophagy–lysosomal pathway in an adenine-induced CKD mouse model. Methods: CKD was induced in C57BL/6 mice by adenine feeding. Mice were randomly assigned to control, control+MSL-7, CKD, and CKD+MSL-7 groups (n = 7–8 per group). MSL-7 (25 mg/kg/day, intraperitoneal; LysoTech, Korea) was administered throughout the experimental period. Renal function was assessed by serum BUN and cystatin C levels. Histological changes were evaluated by PAS and Masson’s trichrome staining and α-SMA immunohistochemistry. Autophagy–lysosomal markers were analyzed using immunohistochemistry, western blot, qPCR, and immunofluorescence. Results: CKD mice showed significant increases in BUN and cystatin C, which were both reduced by MSL-7 treatment. Tubular injury and interstitial fibrosis were markedly attenuated in the CKD+MSL-7 group. p62 expression was significantly increased in CKD kidneys and decreased following MSL-7 treatment. The LC3-II/I ratio was reduced in CKD and showed an increasing trend after treatment. LAMP2 expression, which was decreased in CKD compared to the normal group, was greatly restored by MSL-7 administration. LAMP1 and TFEB expression were also significantly increased in the MSL-7 treated CKD group compared to untreated CKD mice. Conclusion: MSL-7 improved renal function and histological injury in CKD, accompanied by modulation of autophagy-related markers, suggesting a potential association with regulation of the autophagy–lysosomal pathway.
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