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논문분류	춘계학술대회 초록집
제목	Spatial Metabolomic Mapping of Fibrosis-Associated Metabolites in the UUO Kidney
저자	Dazhong Li
출판정보	2026; 2026(1):
키워드	UUO, spatial metabolomics, TOF-SIMS, OrbiSIMS , LC-MS
초록	Objectives: This study aimed to identify fibrosis-related metabolites and visualize their spatial distribution in the kidney using spatial metabolomics in a unilateral ureteral obstruction (UUO) mouse model. Methods: UUO was induced in 8-week-old male C57BL/6 mice by ligation of the left ureter, and kidneys were harvested 7 days after surgery. Untargeted metabolomic profiling was performed using liquid chromatography–mass spectrometry (LC-MS) (sham, n=11; UUO, n=13). Spatial metabolite imaging was conducted using Orbitrap secondary ion mass spectrometry (OrbiSIMS) and time-of-flight secondary ion mass spectrometry (TOF-SIMS). Results: LC-MS analysis identified significant alterations in several metabolites in UUO kidneys compared with the sham group, including increased levels of p-cresol sulfate and trimethylamine-N-oxide (TMAO). Lipidomic analysis revealed revealed increased levels of triglycerides and cholesteryl esters, whereas ceramides, sphingomyelins, phosphatidylcholines, and phosphatidylethanolamines were decreased in UUO kidneys. OrbiSIMS imaging detected 272,404 distinct mass-to-charge (m/z) ion signals across kidney sections, enabling high-resolution spatial metabolite mapping. Phosphatidylethanolamines were predominantly distributed in cortical tubular regions and showed reduced signals in UUO kidneys, consistent with LC-MS findings. In contrast, phosphatidylcholines showed localized increases in specific kidney regions despite an overall decrease observed by LC-MS. Conclusion: Spatial metabolomics using OrbiSIMS and TOF-SIMS enabled high-resolution mapping of fibrosis-associated metabolites in the UUO kidney model. The combined use of LC-MS and SIMS imaging provides complementary information on metabolite abundance and spatial localization, offering new insights into metabolic remodeling during renal fibrosis.
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