대한신장학회

논문분류	춘계학술대회 초록집
제목	Fasudil inhibits tissue factor and plasminogen activator inhibitor-1 secretion by peripheral blood mononuclear cells in CAPD patients
저자	Jiali Wei*, Maowei Xie, Haiyang Huang, Keying Fu, Yun Huang, Wei Wu
출판정보	2016; 2016(1):
키워드	Continuous ambulatory peritoneal dialysis, Fasudil, peripheral blood mononuclear cells, Plasminogen activator inhibitor-1, Tissue factor
초록	Background: Disturbances in hemostasis are common complications of kidney diseases and correlate well with cardiovascular mortality. Little is known about the effects of fasudil on tissue factor (TF) and plasminogen activator inhibitor-1 (PAI-1) expression in peripheral blood mononuclear cells (PBMCs) in CAPD patients. Methods: PBMCs were isolated from 13 individuals with CAPD and 13 healthy subjects. After 4h of incubation with or without LPS (10ng/mL), TF and PAI-1 mRNA of PBMCs were detected by RT-PCR. The levels of TF and PAI-1 in culture supernatants of PBMCs were determined by ELISA. . Results: Compared with healthy controls, CAPD patients had increased TF, PAI-1 protein and mRNA expression by PBMCs at baseline and after stimulated by LPS (10 ng/mL) [P<0.001]. The Fasudil treatment resulted in a significant effect in decreasing TF and PAI-1 [P<0.05] synthesis in PBMCs. TF and PAI-1 mRNA expression and activities in PBMCs were increased in CAPD patients. Conclusion: Fasudil reduced LPS-mediated TF and PAI-1 expression and activity in PBMCs. These effects may partially be relevant to the clinical benefits of fasudil in the treatment of CAPD patients. Table: Table 1. Characteristics of participants TG: triglyceride; TC: total cholesterol; Alb: albumin; Cr: creatinine; SBP: systolic blood pressure; DBP: diastolic blood pressure. Table 2. Medications of 15 CAPD Patients Table 3. Secretion of TF and PAI-1 by PBMCs at baseline and stimulated by LPS. +LPS: stimulated by LPS (10ng/mL). * P <0.001 vs. control spontaneous group. # P < 0.001 vs. CAPD spontaneous group. & P <0.05 vs. CAPD group stimulated by LPS. Fig.1. mRNA expression of TF and PAI-1 in PBMCs. Control indicates normal group; A: RT-PCR analysis of TF mRNA expression (upper bands: GAPDH mRNA,346 bp; lower bands: TF mRNA, 254 bp); B: RT-PCR analysis of PAI-1 mRNA expression (upper bands: PAI-1 mRNA, 596 bp; lower bands: GAPDH mRNA,346 bp); M: Marker; lane 1: normal group; lane 2: CAPD group; lane 3: LPS-treated group; lane 4: LPS and fasudil-treated group. C: determination of relative signal intensity of TF and PAI-1 expression. Bar graph represents quantification of TF/GAPDH or PAI-1/GAPDH mRNA signals of 3 reproductions of independent experiment. For each group, mean ±SD. *P<0.05 health group vs CAPD group. # P<0.05 CAPD group vs LPS-treated CAPD group. & P<0.05 LPS-treated CAPD group vs LPS and fasudil-treated CAPD group. @ P<0.01 LPS-treated CAPD group vs LPS and fasudil-treated CAPD group. Figures:
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