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논문분류	춘계학술대회 초록집
제목	Effect of sorafenib on epithelial to mesenchymal transition of peritoneal mesothelial cells
저자	Joon hyuk SEO, Seok hui KANG, Sae hyun PARK, Kyu hyang CHO, Jong won PARK, *Jun young DO
출판정보	2017; 2017(1):
키워드	sorafenib, peritoneal mesothelial cell, fibrosis, TGF-β, epithelial to mesenchymal transition
초록	Objectives : Epithelial to mesenchymal transition (EMT) of the peritoneal mesothelial cells play a key role in peritoneal fibrosis resulting from peritoneal dialysis (PD). The aim of this study was to evaluate the efficacy of sorafenib on EMT in peritoneal mesothelial cells. Methods : Human peritoneal mesothelial cells (HPMCs) were cultured in a media containing TGF-β with or without sorafenib. For the in vivo study, 20 male Sprague-Dawley rats weight 210−230 g were purchased from the Orient Co. and were divided into 3 groups. The C group received injection of normal saline. The CHX group received chlorhexidine gluconate (CG) every other day for 4 weeks. The CHX+SF group were received CG and cotreated with sorafenib. At the end of 4 weeks, rats were sacrificed for morphometric and histologic analyses. Results : Exposure of HPMCs to TGF-β for 24 h decreased the expression of epithelial cell markers and increased the expression of mesenchymal markers and phosphorylation of Smad2 and Erk. Cotreatment with sorafenib reversed these changes. Macroscopic findings showed that severe inflammatory changes of peritoneum and cocoon formation were confirmed in CHX group. The sorafenib ameliorated these peritoneal changes. Microscopic findings showed that the thickness of the peritoneal membrane in CHX was significantly increased compared with that of the C group. Cotreatment with sorafenib decreased peritoneal thickness and the expression level of the collagen. Conclusions : The present study showed that sorafenib ameliorates the EMT in peritoneal mesothelial cells.
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