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논문분류	춘계학술대회 초록집
제목	Exosomal microRNA signature of obesity using RNA sequencing
저자	Haekyung Lee1, Yunui Bae2, Hyoungnae Kim1, Jin Seok Jeon1, Hyunjin Noh1, Dong Cheol Han1, Yongjin Kim3, Seongho Ryu2, Soon Hyo Kwon1
출판정보	2018; 2018(1):
키워드	obesity \| exosome \| micro RNA \| NGS
초록	Objectives: Obesity is a major risk factor for various diseases such as type 2 diabetes mellitus (DM), hypertension and chronic kidney disease (CKD). Genomic studies have shed light on the pathogenesis of obesity. Exosomes are the intracellular communication tools that carry protein and genetic material including micro RNAs. We sought to identify exosomal microRNAs signature associated with obesity and examine exosomal micro RNA changes after bariatric surgery. Methods: We assessed the microRNA profile of serum exosome from healthy volunteers (HV, n=18) and obese patients (n=16) using RNA sequencing cross sectionally. HV (n=18) and obese patients (n=16) were enrolled prospectively. To evaluate the effect of bariatric surgery, we conducted exosomal microRNA analysis in 4 obese patients 6 months after surgery. Results: NGS analysis identified 224 obesity-related microRNAs (figure 1). Using >2 and <-2-fold changes as cut-offs, we showed that the expression of 72 microRNAs differed between healthy volunteers and obese patients. Biological pathway analysis was suggestive of modulation of signaling pathways including the Wnt pathway, fatty acid biosynthesis, AMPK signaling, p38 MAPK signaling, and AMPK signaling in exosome from obesity patients. Bariatric surgery induced weight reduction led to a marked change in the Wnt pathway and fatty acid biosynthesis pathway. Conclusions: Obesity leads a different microRNA pattern in serum exosome compared to healthy volunteers. Weight loss after bariatric also induces changes in exosomal microRNA profiles. Figure 1
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