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논문분류	춘계학술대회 초록집
제목	Identification of myeloid-derived suppressor cells in type 2 diabetic nephropathy
저자	Jahirul Islam2, Min Ji Kim2, Seung Hee Yang3, Dong Ki Kim1, Yon Su Kim1, Dong-Sup Lee2, Je-In Youn2, Seung Seok Han1
출판정보	2018; 2018(1):
키워드	Diabetic nephropathy \| Myeloid-derived suppressor cell
초록	Objectives: Myeloid-derived suppressor cells (MDSCs) have been known to regulate immune responses in cancer and other pathological conditions. Nevertheless, their expansion in the type 2 diabetic nephropathy has not been evaluated. Methods: Using isolation of peripheral blood mononuclear cells, the proportion of MDSCs was evaluated in 80 patients with non-dialytic type 2 diabetic nephropathy (DN), and compared with those of healthy individuals (n = 11). The DN patients were further divided by the stages of chronic kidney disease. Both phenotypic characterization and functional assay were used to define MDSCs. We then examined the effects of glucose and uremic toxin on the function of MDSCs. Results: MDSCs were categorized into polymorphonuclear (PMN) and monocyte (M)-MDSCs, based on the expressions of CD14, CD11b, CD15, and CD33. The DN patients had a higher proportion of PMN-MDSCs than the healthy individuals, as following median values (interquartile ranges): 2.4% (1.78–3.73%) in healthy individuals; 5.2% (4.44–9.95%) in stages 1–2 DN; 4.5% (2.59–11.03%) in stage 3a; 7.3% (5.39–14.26%) in stage 3b; 8.6% (4.26–12.85%) in stage 4; and 4.2% (3.08–11.98%) in stage 5. However, the proportion of M-MDSCs did not differ between DN and healthy individuals. The Lox-1, a characteristic marker of PMN-MDSCs, was also higher in DN patients than in healthy individuals. The reactive oxygen species production was higher in CD15+Lox-1+ cells isolated from peripheral blood mononuclear cells than in neutrophils, which indicated that these cells contained the functional feature of MDSCs. This trend was increased when the cells were maintained with high concentration of glucose, but not related with the concentration of indoxyl sulfate. The presence of PMN-MDSCs was also identified in biopsied kidney tissues from DN patients. Conclusions: Because MDSCs are expanded in DN patients, their roles in the pathophysiology should be further evaluated.
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